ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (10): 1844-1850.doi: 10.11843/j.issn.0366-6964.2015.10.018

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Effects of PI3K/Akt Inhibitor on Proliferation,Invasion and Apoptosis of DF-1 Cells with Over-expression of Chicken PrPC

WAN Xue-rui,ZHU Man-ling,YANG Run-xia,LIU Gui-lin,LIU Lei,WU Run*   

  1. (College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
  • Received:2015-02-05 Online:2015-10-23 Published:2015-10-23

Abstract:

DF-1 cells with over-expression of chicken PrPC (DF-1-PrP),DF-1 cells with pcDNA3.1(DF-1-NC) and DF-1 cells were used as cell models in order to study the effect of PI3K/Akt pathway on DF-1-PrP cells proliferation,adhesion,invasion and apoptosis and its relationship with ChPrPC expression.After cells were treated with 0,10,20,50,100 and 200 nmol•L-1 Wortmannin,adhesion assay,transwell assay,MTT assay,flow cytometric assay and RT-PCR analyses were used to detect cell adhesion,invasion,proliferation,apoptosis and expression of PRNP mRNA,respectively.The data showed that the expression of PRNP mRNA of DF-1-PrP,DF-1-NC and DF-1 cells were all decreased with the increasing of wortmannin concentration,and adhesion,invasion and proliferation ability were reduced,but apoptosis rate were increased.Furthermore,under the same concentration of less than 100 nmol•L-1,adhesion,invasion and proliferation ability of DF-1-PrP cells was higher than DF-1-NC and DF-1 cells,apoptosis rate was lower.All these results indicated that over-expression of ChPrPC promoted DF-1-PrP cells adhesion,invasion and proliferation and inhibited apoptosis,in which processes PI3K/Akt pathway may play an important role.PI3K/Akt pathway can be effectively blocked by wortmannin,but it may not be the only pathway that ChPrPC regulated cell apoptosis.

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